Inclusive education aims to eliminate barriers in the participation and performance of students, considering their diversity. In this sense, there is a regulation that governs the educational response, being different for each region. Therefore, this study aims to know the attitude of Physical Education teachers of different educational stages in Spain toward students with disabilities. A questionnaire was administered to 272 Physical Education teachers from public schools in a region of Spain. The Mann–Whitney U test was used to determine the relationships between items and dimensions according to sex or center location, and Spearman’s Rho was used to analyze the relationship between dimensions and years of experience. The main results showed that teachers do not feel prepared in terms of training, resources, and infrastructure, although they consider that the integration of students with disabilities in regular classes is beneficial for them.