Environmental assessment in marine systems is difficult because most of the time their preindustrial characteristics are not available. Four sediment cores taken in Mejillones bay (northern Chile) were used to establish preindustrial metals concentrations, and to assess the environmental status of this industrialized zone. According to historical documents, the beginning of the industrial era start in 1850 CE. Considering this, the preindustrial concentration of some metals was established, by mean of a statistical approach. Most of the metals increase their concentration from the preindustrial to the industrial period. Environmental assessment showed an enrichment of Zr and Cr, a moderately polluted condition, and a low probability of toxic effect on the biological communities. The preindustrial values derived from sediment cores are a good tool to evaluate the environmental condition of Mejillones bay. However new information (backgrounds with a higher spatial representativity, toxicological threshold, and others) it is necessary to improve the environmental assessment of this environment.