A checklist compiles the information known about a particular taxonomic group in a specific region. Both the comparison of the sources used, and the definition of the criteria used for the elaborating the checklist make the final result a useful material for decision making on biodiversity conservation. The aphyllophoroid fungi (Basidiomycota, Agaricomycotina) are an artificial category that mainly includes polypores, corticioids and clavarioids. Traditionally, the taxonomy, systematics, and biogeography of the group have been poorly treated at the local level. This paper presents updated data on 16 species of aphyllophoroid fungi from Chile, incorporating new records in new localities, and new data on the ecology of the group. It also adds new information on the recently described species. Species inventories and the constant monitoring of the new data generated on a given taxonomic group, together with the value of biological collections, play an extremely important role in biodiversity conservation.