Aim: To characterize the sealer-dentin interface of hydraulic calcium silicate-based sealers TotalFill and Bioroot and compare it to an epoxy-resin sealer AH Plus. Methodology: An experimental ex vivo study was conducted were 15 single-root extracted premolars were divided into three experimental groups. The teeth were prepared and filled using a single tapered gutta-percha cone. Samples were cut and analyzed using scanning electron microscopy. The images were analyzed using ImageJ software. Results: The median interface value for the three sealers was between 0.6 and 2.5 μm. The largest interface was observed in the TotalFill group in the middle third, and this difference was significant compared to AH Plus (p<0.05). The largest sealer area was for Bioroot in the apical third, but the difference was not significant. Conclusions: Within the limitations of this study, AH Plus had better marginal adaptation in the middle third. The interfacial gaps were similar in the apical third for all sealers.