Stenochiinae is a subfamily of Tenebrionidae with a mainly pantropical distribution, but with a few species distributed at higher latitudes. In general, Stenochiinae has been little studied, and the taxonomic effort been insufficient to understand all its diversity. Recently, a new taxon of Stenochiinae from Central Chile with remarkable morphological characters was collected. Based on phylogenetic analyses of concatenated 28S, 18S, 16S and COI genes and on morphological analyses, a new genus and two species of Stenochiinae are described from Central Chile. Our results suggest that Nestorinus gen. nov. (with the species Nestorinus roitmani sp. nov. and Nestorinus yanettae sp. nov.) has a basal position in Stenochiinae, related to Homocyrtus. The set of morphological characters shared between Stenochiini and Cnodalonini plus their phylogenetic position make it difficult to classify them at tribal level.