Gender stereotypes are the set of social beliefs, attributable to people depending on their biological sex: femininity for them and masculinity for them. The objectives of this study are to know the psychometric properties of the Inventory of Sexual Roles of Bem in a sample of students of Physical Education of Chile and to study the incidence of sociodemographic variables on gender roles in the sample. The sample consisted of 240 students of Physical Education of Chile, to whom this instrument was applied. The results show that the inventory has adequate levels of validity and reliability in the sample studied. Women have a higher level of masculinity and femininity compared to men. Sexual orientation, sex education, religious beliefs or income do not affect the levels of masculinity and femininity in the sample. It is concluded that the instrument is valid and reliable to be applied to this type of samples and that it is necessary to study other sociodemographic aspects that could predict male and female behaviors and attitudes in physical education students.