The study aimed to explore the experiences of perinatal care of lesbian users in the metropolitan area of Chile in 2016. It was a cross-sectional and phenomenological study with snowball sampling. In-depth interviews were carried out and the reports were interpreted using the content analysis technique. The interviews revealed positive experiences by lesbian users during their pregnancy, childbirth and postpartum periods, and an inclusive treatment, with no prejudice by healthcare providers. There is a broad acceptance by healthcare professionals of homosexual families and the visibility of homosexuality. The empowerment and self-esteem exhibited by the interviewed users favor the doctor-patient relationship. However, the alternatives to achieve motherhood are not available for the entire population because of their high cost.