Lake temperature has proven to act as a good indicator of climate variability and change. Thus, a surface temperature analysis at different temporal scales is important, as this parameter influences the physical, chemical, and biological cycles of lakes. Here, we analyze monthly, seasonal, and annual surface temperature trends in south central Chilean lakes during the 2000-2016 period, using MODIS satellite imagery. To this end, 14 lakes with a surface area greater than 10 km(2) were examined. Results show that 12 of the 14 lakes presented a statistically significant increase in surface temperature, with a rate of 0.10 degrees C/decade (0.01 degrees C/year) over the period. Furthermore, some of the lakes in the study present a significant upward trend in surface temperature, especially in spring, summer, and winter. In general, a significant increase in surface water temperature was found in lakes located at higher altitudes, such as Maule, Laja and Galletue lakes. These results contribute to the provision of useful data on Chilean lakes for managers and policymakers.