Abstract A review of the Neotropical Coccinellidae in the Prague Museum, also including to a lesser extent material from other collections, has led to the description of 14 new species from Brazil, Ecuador, Panama, Peru, and Venezuela, two new combinations and 16 first records for countries. The new species described are Cyrea napoensis, Dilatitibialis manaus (Brachiacanthini), Neaporia chucanti, Prodilis saopaulo, P. pastaza and P. qedi (Cephaloscymnini), Siola karpish (Chnoodini), Calloeneis veraguas (Cryptognathini), Diomus chiriqui, D. panamensis and D. sekerkai (Diomini), Toxotoma venezuelae and T. aguascalientes (Epilachnini) and Nexophallobase panamensis (Stilochotidini) nov. spp. The new combinations are Sidonis vianai (for Neorhizobius vianai González, 2013) and Neda areolata (for Neocalvia areolata Gorham, 1982) comb. nov. First country records are Azya exuta Gordon for Ecuador, Chilocorus nigrita (Fabricius) for French Guiana, Harpasus zonatus (Mulsant) for Paraguay, Chnoodes separata Mader for Paraguay, Coelaria erythrogaster Mulsant for Surinam, Sidonis vianai (González) for Brazil, Coleomegilla occulta González for Bolivia, Neda callispilota (Guerín-Méneville) for Uruguay, Epilachna bistrisignata (Mader) for Peru, Epilachna dives Erichson for Bolivia, Hyperaspis pseudodonzeli Gordon & Canepari for Ecuador and French Guiana, Menoscelis saginata Mulsant for Bolivia, Zagloba beaumonti Casey for Bolivia and Venezuela and Scymnus hamatus Gordon for Panama.