Abstract Marketing is a stressful process for beef calves, because they are removed from their environment, often weaned just before loading, loaded, transported, and unloaded. It also involves extended periods without food and water and mixing with unfamiliar animals in an unknown environment. Some studies have shown that calves sold through markets are exposed to extended fasting periods even when they undergo only short transportation times. The aim of this controlled study was to determine the consequences for beef calves of a short transportation time followed by a prolonged time without food and water on their tympanic temperature (TT), maximum eye temperature (MET), blood variables related to stress, and live weight. Ten calves were transported for 3 h and then kept in an outdoor pen for 21 h, completing a 24 h fasting period. Sampling took place before loading, after transport and unloading, and then after completing 24 h without food and water. TT, MET, blood glucose, and creatine kinase (CK) increased significantly after transportation. Live weight decreased across sample times (mean of 10 kg per calf after 24 h of fasting), which was consistent with the higher concentration of -HB found after fasting. Further studies to measure the actual consequences of true, commercial marketing on calf welfare and productivity are needed.