Wildfire induces soil alterations that have a long-term impact on soil organic matter (SOM) quality. We postulated that after different fire severities, the neutral sugars in soils can be used as an indicator of soil organic matter quality after fire. The aim of this study was to determine the contribution of neutral sugar, bulk and occluded particulate organic matter (oPOM) affected by wildfire, at different soil depths in an Araucaria–Nothofagus Forest, four years post-after fire. The concentration and composition of the neutral sugars in the soils clearly comprised the major fraction in the unburned soil. Medium-and high-severity fires caused a drastic reduction in soil sugars in the bulk soil as well as in the oPOM fractions. The13C-CPMAS NMR spectroscopy analysis revealed a high contribution of recalcitrant carbon to the decomposition such as aryl–C and aryl–O derived from charred material, whereas the abundance of O–alkyl C and alkyl C functional groups were decreased. The neutral sugars (Galactose+Mannose/Xilose+Arabinose) revealed a major microbial origin in fire affected areas as the ratio was >2. Therefore. Therefore, we suggest that the neutral sugar content of soil should be used for monitoring both short-and long-term changes in SOM altered by fires.