The media had a stark incluence on rooting with a higher proportion of rooted shoots on WPM and DKW. The highest number of roots per shoot (5.4) and higher root length were observed on WPM. The results stress the importance of evaluating different culture media for specific cultivars under micropropagation. The protocol described permits the micropropagation of blackberry cv. 'Chester', opening a new alternative for plant production in the difficult propagation of this cultivar.