Abstract: Anastrepha fraterculus (Wiedemann) is dominant in the main guava’s crops. Guavas are an important host of A. fraterculus for providing a good performance for the immature and high reproductive capacity. We evaluated the performance of A. fraterculus in two guava varieties related to the fruit physicochemical composition under laboratory conditions. Mature-green guavas of "Tailandesa" (red pulp) and "Kumagai" (white pulp) varieties were exposed to forced infestation by A. fraterculus in laboratory. An additional sample of each variety was submitted to physicochemical analysis. Similar quantities of pupae per fruit (128.5 - 156.0) and pupal viability (85.9% and 87.5%) were obtained per variety, but "Kumagai" produced significantly more pupae of A. fraterculus per fruit mass (1000.6 pupae/kg). Pupal weight was higher in "Tailandesa" (115.3 mg/10 pupae) than "Kumagai" (82.6 mg/10 pupae). Significant positive correlations were obtained between pupae per fruit or kg versus adults per kg for both varieties. A positive correlation was observed between pupae per fruit and pupae per kg of "Kumagai" guavas. Peel and pulp firmness were inversely correlated with pupae per fruit and pupae per kg. Weight of 10 pupae of A. fraterculus was correlated with fruit weight of "Tailandesa" guavas. The multivariate analysis PCA showed a relationship between pupae per kg and peel firmness, pulp firmness, Ratio, soluble solids, and titratable acidity in "Kumagai" guava. This explains the higher infestation index pupae per kg and better reproductive performance of A. fraterculus in "Kumagai" guava.