The remnants of coastal Mediterranean sclerophyll forests in Chile have a low representativeness in the National System of Protected Wild Areas despite their high endemism and species richness. In the Biobio Region, the Hualpen Terrestrial Biology Station (EBT) protects a remnant of this type of forest, for which there are no revisions describing its flora, which makes it difficult to formulate and develop strategies for its protection and conservation. This work aims are: a) to describe the taxonomic composition of vascular plants of the ETB and b) to provide information on their geographical origin, habit and conservation status. The resulting floristic list includes species identified through systematic sampling in the study area for one year, as well as EBT species stored in the collection of the Universidad de Concepcion Herbarium. We identified 292 species of vascular plants, where 71 species are endemic, 124 are native, 99 are alien species. From the floristic point of view the Asteraceae family was the most diverse with 36 species, followed by Poaceae with 32 species and Fabaceae with 18 species. Herbs were the most frequent growth habit (77.2%), followed by shrub (13.9%), tree (7.5%) and sub-shrub (1.4%). Nineteen endemic and native species have been classified in some category of conservation in continental Chile, standing out Gomortega keule and Pitavia punctata both classified as endangered species, and the fern Asplenium trilobum classified as a least concern in Chile and vulnerable in the Biobio Region. Despite of the reduced protected area in the EBT (70 ha), the site has a high species richness of native species when compared with data obtained from other studies of flora in sites of central Chile.