Numerous studies agree that the beginning of university life is associated with unhealthy changes in eating practices and lifestyles that can be consolidated in adulthood. The aim of this study was to assess the extent to which the nutrition and physical activity of university students from three career programs comply with the recommendations of the new Dietary Guidelines for the Argentine Population (2016). One hundred and sixty-four (164) students of Biochemistry (BQ), Bachelor of Nutrition (LN) and Bachelor of Biotechnology (LB) of the Universidad Nacional del Litoral were surveyed. LN students approached the recommendations more than BQ and LB students: a higher percentage had four meals per day (45.0% LN vs. 22.4% LB and 21.8% BQ, p-value=0.009) and consumed a daily portion of milk/yogurt (p-value=0.042); the BQ students had the lowest fruit consumption (p-value=0.000), while the LB students had the lowest vegetable intake (p-value=0.023). All students presented patterns of intake and consumption of foods strongly influenced by the Argentine culture. Almost eighty percent (79.3%) of students did physical activity, but only 59.8% reached the recommended 30 minutes a day. The University offers healthy eating spaces (university canteen) and physical activity (sports grounds) that were not sufficiently exploited. It is concluded that it is necessary to plan interventions to promote and / or improve patterns of food consumption and physical activity, according to healthier lifestyles for university students.