Introduction: The correct determination of the Vertical Dimension (VD) is essential when rehabilitating a patient. There is little research in the literature that collectively relates the VD to other variables such as electromyographic activity. The objective of this study is to describe a technique for the analysis of the VD through the Interocclusal Distance, using electromagnetic articulography and surface electromyography. Material and Methods: The VD was recorded by using an AG501 electromagnetic articulgraph simultaneous-ly with the electromyographic activity of masseter and temporal muscles using EMG VIII surface electrom-yograph, according to DVO and DVP protocol. The study was carried out in the Oral Physiology Laboratory of the Center for Research in Dental Sciences (CICO) with the approval of the Scientific Ethical Committee of the University of La Frontera. Results: A protocol was obtained for the use of electromyography and 3D electromagnetic articulography in the vertical dimension analysis, which allows the vertical dimension to be characterized by a vertical distance measurement, 3D distance measurement, opening angle and the electrical activity of the lifting muscles of the jaw to different degrees of opening. Conclusion: The proposed protocol could be used to generate clinical recommendations to rehabilitate patients.