Objective: To determine the psychometric properties of the Spanish version of the Jeffries questionnaire to assess perception, satisfaction and learning results in Clinical Simulation (QJSC) for students of Health Science degrees. Method: For the transcultural adaptation to the Spanish language of the QJSC questionnaire, the inverse method was applied with a back translation. Internal consistency analysis was performed to assess reliability, analysed with Cronbach's alpha, a value between 0.90 and 0.95 was considered acceptable. To establish the content validity, a factor analysis was performed, only items with factor saturations greater than 0.60 were considered for interpretation. Statistical analyses were performed with the SPSS 19 programme. The administration of the questionnaire was carried out in 10 to 12 clinical simulation training sessions according to the subject programme with 10 to 12 students per session, for high-fidelity clinical simulation between 2017 and 2018, in a sample of 443 undergraduate students of the Health Sciences degrees corresponding to Nursing, Nutrition and Dietetics, Medical Technology and Kinesiology in the Clinical Simulation Centres of the Coquimbo region, La Serena, Chile. Results: The internal consistency level for QJSC was Cronbach's Alpha = 0.963. The data fulfilled the Kaiser-Meyer-Olkin criteria for adequate sample MSA (measure of sampling adequacy) of 0.981 with an acceptable minimum of 0.500. The data also met the requirements of the Bartlett sphericity test (χ2 = 53971, df = 561, p <.0001). The questionnaire showed a factorial structure of two factors which were named Active-Participatory Learning and Perception of Autonomy in learning. Conclusions: It was concluded that the evidence on the validity and reliability of the scores for the Spanish version of the QJSC indicates that it is an adequate tool to evaluate the perception, satisfaction and learning outcomes of students in this teaching and learning methodology.