The association between obesity and an increased risk of cardiometabolic morbidity and mortality is a fact, but most of the time there are metabolic disorders that cause autonomic changes not always detected by health institutions. Objective: To demonstrate the effect of a high intensity and short duration interval protocol on cardiovagal activity in overweight people through the evaluation of heart rate variability. Method: a total of 20 overweight people without other morbid backgrounds collaborated in the study. Their age was 24.6 +/- 2.17 years old; body mass index (BMI) of 27.11 +/- 1.38 kg.m-2; systolic and diastolic blood pressure of 126.4 +/- 2.49 and 86.1 +/- 1.85 mmHg, respectively. Sampling was done by convenience. They were randomly distributed in Intervention Group (GI, n = 10) and Control Group (GC, n = 10). Results: Significant differences were registered between the groups, the starting values being lower than after sixth week (F: 120,412; G.L. = 1; 7; p = 0,003). In addition, there is a statistical difference between the SD1 value of the GC>GE (F: 6,700; GL = 1; 7; p = 0.036) and only in the GI, between 1 week>6 weeks (EMMEANS = 10,400; p = 0.001). Conclusion: After the six-week intervention with a vagal activation protocol, parasympathetic modulation at rest increased in overweight people.