The purpose of this article was exploring the role of discursive and non-discursive symbolization - specifically gestures - in the negotiation of differences in couples ' interactions. Five married heterosexual couples were invited to hold a conversation about an unsolved problem in their relationship. A videographic analysis was carried out to explore gestures in dialogical sequences and Microgenetic Semiotic Analysis (ASM) was conducted. The results showed that gestures complemented verbal signs as semiotic devices in the regulation of meaning construction and differences in negotiation, displaying strategies for facing and avoiding conflict, as well as resources for undergoing tension dealing with personal objectives and emotional difficulties; Its role is discussed as preparation of the verbal communicative expression, as devices for self-regulation and access to tension relief. Further, the discussion addresses their function as paths for encounter, mutuality and closeness.