The following article presents the formulation of the concept of regional cultural identity, based on a selection of the mental aspect of culture, which resulted in a narrative discursive definition. For this, metaphysical, linguistic and historical foundations are presented, and a contextualization composed of definitions of cultural identity, Latin American cultural identity and regional cultural identity. Regarding the latter, applications to the study of the Cultural Identity of the Region de los Rios are presented, due to the characteristics of this territory as a new region. The resulting concept describes the phenomenon as a sense of the regional being present in the cultural semiosphere, forming part of the collective memory and imaginary, manifested in the way of the thematic unity of a coherent and ordered historical narrative, progressively more complex and heterogeneous, which combines the linear progression of the subthemes and the constant theme of regional cultural identity.