The PowerPlex ESI 17® (PP17) amplification kit was designed for European population (ENFSI and EDNAP recommendations). Routinely, DNA testing in our population was performed using the AmpFℓSTR® Identifiler® PCR Amplification kit (ID). Our population is an admixture between Hispanic and Amerindians. To evaluate the usefulness of the loci included in PP17 we analyzed 150 unrelated individuals' samples obtained with previous informed consent, who attend to paternity testing. The DNA extraction was automated performed and the STR loci was PCR amplified using PP17 kit. Allelic, genotypic frequencies, Power of exclusion (PE), Match Probability (MP) and polymorphism information content (PIC) were calculated using PowerStat v12 software (Promega). This study showed a high percentage of heterozigosity in PP17 loci in the population studied, despite being designed for European population, showing that PP17 system has better informativeness than ID. The combined use of these two systems could resolve complex DNA testing, where ID alone is not sufficient to get a strong conclusion. © 2011 Elsevier Ireland Ltd.