Objective. To assess the prevalence of antibodies to Trypanosoma cruzi (T. cruzi) in a sample of domestic dogs living in two endemic municipalities. Materials and methods. Serum samples were taken from 20 dogs from households with seropositive pregnant women, and 40 dogs from households with seronegative pregnant women in Miraflores and Moniquira, Boyaca. The analysis was carried out through INBios RDT dipstick. Results. A prevalence of 16.7% and 13.3% in Moniquira and Miraflores respectively was found, with an overall prevalence of 15% in the two municipalities. There is a risk of acquiring infection in dogs three times greater in households with sero positive pregnant women. Flea and tick infestation in the animal, and habitat near the house, were associated with higher prevalence in the canine. Conclusions. Breed, gender, presence of birds in the house and clinical examination are considered prognostic factors for the infection by Trypanosoma cruzi in canines. As protection factors de-worming and vaccination of animals were identified.