The emissions from a vehicle using a characteristic diesel fuel for Chile and blends B2 and B5 with biodiesel from recycled vegetable oil were measured. Experiments were carried out using a test bench consisting of a roller dynamometer and a diesel vehicle. A combustion gas analyzer was used to determine the concentration of CO, CO2, NOx and particulate matter (concentration and size distributions). Both the particle size distributions and concentrations of NO, NOx and CO varied based on the speed of the vehicle during the driving cycle, indicating that the gas emissions were dependent on the instantaneous air/fuel ratio and the fuel used. Calculated emission factors showed that CO, CO2 and PM10 emissions had no significant differences with the use of biodiesel blending and NOx emission increase from B5. The results obtained were preliminary data on the implementation of a project (FONDEF D09-I-1070) aimed at generating a database which correlate energy efficiency and pollutant emissions of biofuel from different origins and their blending with fossil fuels. This is an abstract of a paper presented at the 106th AWMA Annual Conference and Exhibition (Chicago, IL 6/25-28/2013).