Background.Today, physical inactivity in Chile, rather than a fashion aspect is a serious health problem associated with the development of chronic non-transmissible diseases. Objective.To determine the effects of a two-month overload exercise program up to muscular failure on the lipid profile of sedentary adults. Materials and methods. An experimental study type with pre-test and post-test carried out on a sample of 16 subjectsdivided into two groups. Group Adherent (GA, n = 8, age = 34.88 + 6.89 years) and Group non-adherent (GNA, n = 8, age = 35.13 + 6.64 years). The sample is a non-probabilistic type, with subjects chosen for convenience in a non-random way. GA participants underwent physical resistance exercise up to muscular failure twice a week during two months. Total cholesterol (total-Col), HDL cholesterol (HDL-C), LDL cholesterol (LDL-C) and triglycerides (TG) were evaluated in both groups (fasting ≥12 hours) before and 72 hours after the last intervention session. Results. After the intervention there were not significant differences (p> 0.05) in total-Col, HDL-C, LDL-C and TG. Conclusions. While a downward trend in the lipid profile is noted, a longer duration and/or more frequent intervention may be needed to significantly alter the plasma variables.