The aim was to determine the acute effects of high-frequency electrical stimulation (HFES) on the morphohistology and desmin content of the soleus, tibialis anterior (TA) and extensor digitorum longus (EDL) muscles in old rats submitted to hindlimb suspension (HS). Male Sprague-Dawley rats (23 months-old) were submitted to HS for 14 days. After this period, the Control and HS groups underwent a single session of HFES. HS decreased muscle mass and CSA-MF in all the muscles and desmin content in the soleus and EDL muscles, but increases its content in the TA muscle in the control group. HFES increased the CSA-MF in the soleus muscle and decreased it in the TA and EDL muscles in the control group. HFES also increases the desmin content in the soleus and EDL muscles, but decreases it in the TA muscle. One session of HFES has different effects on the morphohistology of the soleus, TA and EDL muscles in this study.