Acidiphilium sp. DSM 27270 (Yenapatur) forms part of a microbial consortium isolated from copper mines in Chile that is currently used in state of the art industrial-scale biotechnology. Its taxonomic assignment is still tentative and is metal resistance and homeostatic responses poorly characterized. In this work the genome of Yenapatur was re-assembled, annotated and compared to other sequenced strains of the genus. Genomic signatures were derived to better define the taxonomy of the strain. The minimum inhibitory concentrations of diverse cations and anions were determined. Known resistance determinants were profiled in the genome of Yenapatur and publicly available sequenced Acidiphilium strains. Results are presented and discussed under the light of the operational conditions in which Yenapatur thrives.