Flotation tailings are hazardous due to their physical and chemical instability and are considered one of the main pollution concerns of the mining industry. Most of these tailings are fine-grained residues with low copper content (0.1-0.8%) and include toxic compounds, such as heavy metals, arsenic, and flotation reagents, amongst others. The generation of acid mine drainage (AMD) due to biological oxidation of tailings represent another environmental concern. On the other hand, low-grade ore as copper source is challenging the mining industry to commercially produce this metal. The main objective of this work was to assess the use of flotation tailings and low-grade ore to biologically produce copper sulfide for further pyrometallurgical or fine chemistry processing. Low-grade ore and flotation tailings were first agglomerated at different proportions and leaching tests were performed in 1 m columns, using either industrial or synthetic raffinate solution. Then, the pregnant leach solution (PLS) obtained from the column tests was subjected to selective precipitation of copper by using biogenic hydrogen sulfide (H2 S) generated from a mixed culture of neutrophilic sulfate reducing bacteria (SRB), enriched from sediments of the Caracarani river (located at 4,600 m above sea level in northern Chile). Over 91% of the soluble copper present in the PLS was recovered as copper sulfide in 28 days, whilst ferrous iron, other metals and arsenic remained in solution. Analysis performed by X-Ray diffraction confirmed the presence of copper in the precipitate (58% as covellite). This work demonstrates the possibility of applying bioleaching and bio-precipitation technologies to treat tailings and low-grade copper ore to produce high quality copper sulfide.