Nurses are on the front line of the healthcare system and should, therefore, have the evidencebased knowledge to manage breastfeeding. The objective of this study was to assess the attitudes and knowledge about human lactation among a group of nursing students. An anonymous online survey was sent to all College of Nursing students at a local university in Kentucky. Nursing students participating in the survey favored breastfeeding over formula feeding for infants. Lack of knowledge and various misconceptions about breastfeeding were reported. Whether a nursing student has or has not completed a class about human lactation during the nursing program, and has had or not had children, significantly influences their attitudes and knowledge toward breastfeeding. Future studies should focus on identifying if nursing faculty members and nursing students recognize human lactation as a value and central knowledge for nurses.