The conceptual dimension of knowledge is understood as the «knowing what», usually communicating or teaching from different school disciplines, except in physical education. The objective of the present investigation is to understand the knowledge in physical education from its conceptual dimension expressed by the students of Chile and Colombia and to establish the differences that exist in the variables (gender, country, and age). The study was methodologically descriptive and cross sectional using a single measurement. For this purpose, a psychometric instrument was created and validated. The results indicate that only 21.3% in the case of Chile and 9.5% in Colombia managed to surpass the average score of the questionnaire (16 points). These results demonstrate a low culture of conceptual knowledge that the students of Chile and Colombia have.