The feline herpesvirus type 1, generates multiple problems and the cat ends with consequences that affect its futurquality of life. This virus is distributed throughout the world and is easily transmitted and since it is a latent pathogenit continues to spread uncontrollably to the entire cat population. The diagnosis is based on clinical signs, today theris only a specific laboratory diagnostic method implemented in Chile to identify the agent, which is not used regularlin the clinic of small animals. Thus, the treatment and diagnosis are usually based on the knowledge and experience oveterinarian without leaving a real confirmation about which agent is causing the symptoms. This research proposea molecular diagnostic method alternative to timidine kinase detection gene, for which cats under 1 year of age werselected, with symptoms compatible with an infection with the feline herpesvirus. The Polymerase Chain Reactio(PCR) method was used to detect the feline herpesvirus type 1 glycoprotein B gene, followed by the determinatioof the percentage of nucleotide identity (NIP) from official GenBank® data. Of the 11 cats studied, only one of them resulted in the amplification of a segment corresponding to the glycoprotein B gene. The resulting NIP (> 96%) confirmthat the sequence obtained corresponds to type 1 glycoprotein B gene of feline herpesvirus and the efficiency of thmethod implemented is discussed.