Several plants of potato, cactus pear and carrot showing symptoms of presumable phytoplasma infection, were collected in Metropolitana and Lagos regions of Chile. Nucleic acid was extracted from the leaf midribs and used for phytoplasma detection. The amplification products of the expected size with nested polymerase chain reaction on tuf and 16S rRNA genes were obtained only from symptomatic samples. Cloning, sequencing and virtual restriction fragment length polymorphism analysis, allowed the identification of the 16SrIII-J phytoplasma presence in all plant species tested.