This work reports the optical, electrical and morphological properties of graphene (G) films. Firstly, glass substrates were coated with iron oxide (IO) films using a spraying method. Subsequently, the sputtering technique was employed for the formation of graphene films on the IO layers (G/IO films). According to XPS spectra, the IO films offered anchorage sites for the growth of G that improved the adherence to the substrate. Additionally, the sheet resistance of the films was in the range of 16-19.5k/. Also, the optical measurements revealed that the G/IO films subjected to a post-deposition heat treatment presented a transmittance similar to 11% higher than those as-deposited. In fact, those heat-treated films presented a transparency above 80% for wavelengths above 850nm and a high elasticity modulus of 0.188 +/- 0.039 TPa, which make them good candidates to be used as transparent conductive electrodes in thin film transistors, surface plasmon biosensors or surface enhanced Raman scattering (SERS) substrates.