The last national survey on seroprevalence of T. cruzi human infection in Uruguay, showed a 3.4% in adults from endemic areas. Since 1983, antivectorial actions of the control program have been carried out continually. In consequence, household infestation by Triatoma infestan, the main vector of T. cruzi in Uruguay, decreased in all endemic areas and was completely eliminated in some of them. The objectives of the present work are to evaluate the new seroepidemiological situation. A representative sampling of rural and urban population was undertaken, to include six and twelve year-old schoolchildren from three departments: Artigas, Rivera and Tacuarembo. The whole sample included 4,722 schoolchildren, evaluated by the indirect inmunofluorescense (IFI) test for Chagas<FONT FACE=Symbol>¢</FONT> disease. The seroprevalence of T. cruzi infection in the six year-oldgroup was 0.3% in Artigas, 0.6% in Rivera and 1.0% in Tacuarembo. The seropositive children with seropositive mothers support the possible congenital transmission.