Objetive: To identify the frequency of infections related to Central Cenous Catheters (CVC)in children and determine if the colonization of the line predicts infection. Patients and Methods. A longitudinal descriptive study of 109 CVC. Colonization was detected at the entry point and connection points of the lines with a semi quantitative method. Definitions were made according to CDC (Atlanta) Criteria. Results: The incidence of infection into the blood stream was 11%, density of incidence 9/1000 days/ catheter. Risk factors were identified: a) surgery (RR 4,2 IC95% 1,5-11,7), b) ostomies (RR 4,0 IC95% 1,4-11,4), and c) colonization in line connections (RR 3,9 IC95% 1,2-12,3). Agents identified were Staphylococcus coagulasa (-) 83,4% and Candida albicans 16,6%. Incidence of local infection was 5,5%. Sensitivity, specificity and (+) predictive value of cultures of cultures and connections were very low. Conclusions: In this experience, the incidence of infection associated to peripheral lines was quite high. Colonization of insertion and connections does not predict infection. The implemmentation of Bundle is recommended to prevent these infections.