The current study was carried out with the purpose of evaluating two alternative diets in the feeding of Colossoma macropomum alevins using earthworm flour (Eisenia foetida), soybean (Glycine max) and beans (Phaseolus vulgaris) as the protein source and as fishmeal substitute and was compared with a control diet. The diets were formulated with a crude protein with a theoretical 32% protein. The body composition of C. macropomum fed the different diets showed that protein was between 47,3%% and 48,6% and the lipid content was found between 21,2% and24,3% not showing significant differences between diets (p>0,05). According to the results obtained there is a reason to think that a total substitution of fishmeal by earthworm meal, soybean and beans would be successful in feeding C. macropomum alevins.