Additions of new species of herpetological fauna to the Herpetological Collection of the Museo del Mar, Universidad Arturo Prat, Iquique, Chile, are documented. The Collection currently contains 56 herpetological species, 16 of which are Amphibia, and 40 of which are Reptilia, both terrestrial and marine. There is a total of 165 specimens, of which approximately 38% are Chilean species