Nothofagus alessandrii is an endemic and endangered species from the mesomorphic zone of Chile. Currently, differences across populations are not well known. To this end, quantitative and qualitative parameters pertaining to germinative material and nursery performance of seedlings from five provenances representing the natural distribution of the species were analyzed and characterized. Seeds were collected in February 2000 and size, form, weight, and germination capacity were determined. Seedlings were then cultivated in nursery in September of the same year, and basal diameter and height growth were quantified. Results indicate that seed size is generally homogeneous across sites, and statistical differences were limited to length of dimerous seeds and width of trimerous seeds. Seed weight and germination capacity varied significantly among the provenances. After one cultivation season, the development of the nursery plants was homogenous across provenances, with no differences in basal diameter or height. N. alessandrii differs, in relation to the variation among the studied provenances, from that of other species of the same genus, with the exception of a pronounced clinal type. However, the southern-most provenance tended, in general, to differ from the other ones and likely represents a different ecotype.