The greater palatine foramen (GPF) conducts the greater palatine nerve, responsible for the innervation of the posterior hard palate. The morphometric knowledge of the GPF is essential in the anesthetic interventions required in maxillofacial surgical procedures. The aim of this study was to evaluate the location and shape of the GPF in dry skulls of adults from Southern Brazil. Male (n = 65) and female (n = 29) skulls were studied, and the distance measurements from the middle of the GPF to the mid-sagittal plane (MSP) and to the posterior border of the hard palate (PBHP) were analyzed using a digital caliper and a ruler. The mean GPF-MSP distance in the right and in left sides of male skulls were 15.6 mm and 15.4 mm, respectively, and in female skulls, 15.63 mm in the right side and 15.47 mm in the left. The mean GPF-PBHP distance on the right side was 3.5 mm in male skulls, and 3.1 mm in female skulls, and on the left side, 3.6 mm and 3.2 mm in male and female skulls, respectively. Student's t-test showed no significant difference in the GPF-MSP and GPF-PBHP mean distances when compared to gender and to the left and right sides. The predominant GPF shape was ovoid, followed by lancet or slit, and round. This study provides comparisons of ethnic data, which may help clinically in oral and maxillofacial anesthesia.