A total of 189 faecal samples of the critically endangered Darwin's fox (Pseudalopex fulvipes [Martin, 1837]), from different areas within Chiloe Island in southern Chile were examined for parasites eggs and oocysts using the sugar flotation technique. The results showed that 21.2% of the samples were positive to either one of nine helminthes and one protozoan. The parasites with the highest prevalence were an ascarid nematode and a Spirometra species of cestode. Other identified endoparasites include Capillaria sp., Toxocara canis,Toxascaris leonina, Filaroides osleri, ancylostomatid nematodes. Trichuris sp., Taenia sp., and Isospora sp. Parasite loads were higher during the winter and in areas having more domestic dogs. All these species and genera are reported for the fist time in the Darwin's fox.