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Isolation and characterization of ten microsatellite loci in the catfish Trichomycterus areolatus (Siluriformes: Trichomycteridae), with cross-amplification in seven Trichomycterinae species
| Indexado |
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| DOI | 10.1007/S12686-011-9569-2 | ||||
| Año | 2012 | ||||
| Tipo | artículo de investigación |
Citas Totales
Autores Afiliación Chile
Instituciones Chile
% Participación
Internacional
Autores
Afiliación Extranjera
Instituciones
Extranjeras
Abstract
A total of ten microsatellite loci were isolated and characterized in the endangered freshwater catfish Trichomycterus areolatus from Chile. Samples from three separate watersheds were also analyzed. The mean number of alleles per locus in a sample of 63 individuals was 4 (1-13 alleles per locus) and the observed heterozygosities ranged from 0 to 0.83. Hardy-Weinberg equilibrium was observed in all loci except for a few isolated cases, while none of the loci exhibited significant linkage disequilibrium. Cross-amplification analysis showed a low number of loci and alleles amplified in other seven species of the Family Trichomycteridae.
| Ord. | Autor | Género | Institución - País |
|---|---|---|---|
| 1 | Munoz-Rojas, Pablo | Hombre |
Universidad de Chile - Chile
|
| 2 | Quezada-Romegialli, Claudio | Hombre |
Universidad de Chile - Chile
|
| 3 | VELIZ-BAEZA, DAVID ENRIQUE | Hombre |
Universidad de Chile - Chile
|
| Fuente |
|---|
| FONDECYT |
| CONICYT |
| CONICYT, Chile |
| Fondo Nacional de Desarrollo Científico y Tecnológico |
| Comisión Nacional de Investigación Científica y Tecnológica |
| Comisión Nacional de Investigación CientÃfica y Tecnológica |
| Fondo Nacional de Desarrollo CientÃfico y Tecnológico |
| IEB |
| Agradecimiento |
|---|
| We thank C. Newton and ATG Genetics, M. Espinoza for lab assistance and L. Eaton for reviewing the English version of the manuscript. This study was supported by Fondecyt 1100341 to DV. DV also thanks Grant PFB-23 (CONICYT, Chile) and Grant ICM P05-002. PM thanks IEB master grant and CQR thanks CONICYT doctoral grant. |
| Acknowledgments We thank C. Newton and ATG Genetics, M. Espinoza for lab assistance and L. Eaton for reviewing the English version of the manuscript. This study was supported by Fondecyt 1100341 to DV. DV also thanks Grant PFB-23 (CONICYT, Chile) and Grant ICM P05-002. PM thanks IEB master grant and CQR thanks CONICYT doctoral grant. |
