EPR measurements have been performed on single crystals of [Cu(L-tyrosine)(2)](infinity) at 33.8 GHz and at room temperature. The EPR spectra display partially resolved EPR lines for most orientations of the magnetic field in the ab plane, and only one resonance for orientations close to the crystal axes, while only a single line is observed along any direction in the ca and cb crystal planes. This behavior is a result of the selective collapse of the resonances corresponding to the four copper sites in the unit cell produced by the exchange interactions between the copper ions. The magnitudes of the exchange interactions between the copper ions were evaluated from the angular variation of the line width and the collapse of the EPR lines. The value vertical bar J(AD)/k(B)vertical bar =0.8 K between neighboring copper atoms at 4.942 angstrom is assigned to a syn -anti equatorial -apical carboxylate bridge with a total bond length of 6.822 angstrom, while the small value vertical bar J(AB)/k(B)vertical bar = 0.004 K is assigned to a long bridge of 11 atoms with a total bond length of 19.186 angstrom, that includes one resonance assisted hydrogen bond (RAHB). This finding is discussed in terms of values obtained for similar paths in other model compounds and in proteins. (C) 2012 Elsevier Inc. All rights reserved.